Fig. 6. Ceramide regulates peritoneal macrophage IL-10 production through p38 signaling. Peritoneal lavage (PL) macrophages were pre-treated with either AT or ceramide and stimulated with LPS. A) The percentage of IL-10 producing macrophages was quantified. * p<0.05 vs +LPS; # p<0.05 vs +LPS+Cer; n = 4-8 mice/group, as well as B) phospho-p38 levels in macrophages. * p<0.05 vs +LPS; # p<0.05 vs +LPS+Cer; n = 4 mice/group. C) The percentage of IL-10 producing macrophages was measured and compared when treated with AT compared to LPS alone or LPS and selective p38 inhibitor SB239063. * p<0.05 vs +LPS; # p<0.05 vs +LPS+SB; n = 4 mice/group. The gating strategy is the same as shown in Fig. 5. One representative histogram per group is shown.